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Sorry if I gave the impression that I grew it from seed. My green fingers only go so far. A friend who is much more patient grew them from wild seeds and donated a whole load of pink and white ones to the Manada Conservancy for their spring wild flower sale.
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Nikon D850 with a Sigma 135mm f/1.8

0.5s @f/7.1 ISO64

 

I probably would have used a RRS tripod with BH-55 ballhead.

 

I used the built in Focus Shift feature but I don't remember exactly what settings I used, sorry. The 45 shots were stacked using Helicon Focus and I believe I used the Method B with a Radius of 8 and a Smoothing of 4.

 

For those interested, Focus Shift using the more recent Nikon's is really easy. You focus on the nearest point and specify how many shots you want to take (max 300) and the Focus Step Width which is how much the lens automatically shifts for each image. Press the go and wait for it to finish, if it reaches Infinity before the number of images is reached it stops automatically.

 

Heres a useful tutorial on the Focus Shift feature.

 

D850 TIPS - Focus Stacking (Focus Shift Photography) | Technical Solutions | Nikon Professional Services

 

Focus Stacking can be achieved with manual lenses by physical altering the focus between shots, for this you need a really solid mount and ideally a cable release,

 

Heres an early attempt from a few years ago using a manual lens. This was only 13 shots and again stacking in Helicon Focus.

 

http://1.bp.blogspot.com/-Qdj83G5VTyk/U_PPTa6ufSI/AAAAAAAABCs/r68xXJkZgeo/s1600/Rain%2BLily%2B2.jpg

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