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I am using epsom v550. I had good scans a few days ago but now with same negative I get poor quality and colored lines.and very dark scans. Also the raw scan was inverted color and that isn't the case now. I use Vuescan and I did not alter settings that I know of. Am I missing something or is scanner in need of maintainence or replacement or the vuescan need to be reinstalled?issue.thumb.jpg.4e07631f5176c3e0b64275b076ac9a67.jpg
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The Scanner probably needs cleaning. If there are any mirror/s, and they have never been cleaned, it's time to clean them now. If you can get to the scanner's lens, clean that too. That cleaning should eliminate the banding and maybe even correct the color cast

 

You could then try reinstalling Vuescan but get the latest version that suits the V550

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